メダカ (Oryzias latipes)

• ＜卵母細胞の成熟の分子メカニズムの解明＞脊椎動物の卵母細胞は第一減数分裂の中期で細胞周期が停止した状態で成長を続け、脳下垂体から分泌される生殖腺刺激ホルモンのはたらきで減数分裂が再開されると受精能を獲得します。現在の卵母細胞を取り囲む濾胞細胞で合成される卵成熟誘起ステロイドの合成経路やその反応系の解明を目指しています。
• ＜受精反応によって起こる多精拒否の分子メカニズムの解明＞受精時に卵から分泌される物質によって引き起こされる卵膜の変化を調べ、2番目以降の精子が卵子に接触できなくなる仕組みを明らかにすることを目指しています。

• 2000年 3月　博士 (理学) (北海道大学)　(学位論文: Studies on the chorion hardening upon fertilization in medaka (Oryzias latipes) eggs)

• 1999年 3月　北海道大学大学院 理学研究科 博士課程 単位取得退学

• 2000年 4月　岡崎国立共同研究機構 基礎生物学研究所 生殖生物学研究部門 COE非常勤講師
• 2001年 4月　日本学術振興会 特別研究員（基礎生物学研究所）
• 2004年 4月　独立行政法人科学技術振興機構 研究員（基礎生物学研究所）
• 2005年 4月　自然科学研究機構基礎生物学研究所 契約職員（研究員）
• 2011年 3月　米国 The University of Washington, School of Aquatic and Fishery Science, Visiting Scientist
• 2015年 4月　東京学芸大学 教育学部 自然科学系生命科学分野 個人研究員
• 2016年 4月　帝京科学大学 生命環境学部 生命科学科 准教授

• Shibata, N., Nakamoto, M., Shibata, Y., Nagahama, Y. Endocrine regulation of oogenesis in the medaka, Oryzias latipes. In: Medaka: A model for organogenesis, human disease, and evolution. Naruse, K., Tanaka, M., and Takeda, H. (Eds.) Springer Link. (pp.267-283). 2011.（書籍・分担執筆）
• Shibata, Y. Modification of egg envelope proteins during fertilization in teleost fish. In: New impact on protein modifications in the regulation of reproductive system. Tokumoto, T. (Eds.) Transworld Research Network, Kerala, India. (pp.115-134). 2005.

• Fairgrieve, M.R., Shibata, Y., Smith, E.K., Hayman, E.S., Luckenbach, A.J. Molecular characterization of the gonadal kisspeptin system: cloning, tissue distribution, gene expression analysis and localization in sablefish (Anoplopoma fimbria) Gen. Comp. Endocrinol. 225, (212-223). 2016.
• Paul-Prasanth, B., Bhandari, R.K., Kobayashi, T., Horiguchi, R., Kobayashi, Y., Nakamoto, M., Shibata, Y., Sakai, F., Nakamura, M., Nagahama, Y. Estrogen oversees the maintenance of the female genetic program in terminally differentiated gonochorists. Sci. Rep. 3, (2862). 2013.
• Shibata, Y., Iwamatsu, T., Suzuki, N., Young, G., Naruse, K., Nagahama, Y., Yoshikuni, M. An oocyte-specific astacin family protease, alveolin, is released from cortical granules to trigger egg envelope hardening during fertilization in medaka (Oryzias latipes). Dev. Biol. 372, (239-248). 2012.
• Chakraborty, T., Shibata, Y., Zhou, L.Y., Katsu, Y., Iguchi, T., Nagahama, Y. Differential expression of three estrogen receptor subtype mRNAs in gonads and liver from embryos to adults of the medaka, Oryzias latipes. Mol. Cell Endocrinol. 333, (47-54). 2011.
• Shibata, Y., Paul-Prasanth, B., Suzuki, A., Usami, T., Nakamoto, M., Matsuda, M., Nagahama, Y. Expression of gonadal soma derived factor (GSDF) is spatially and temporally correlated with early testicular differentiation in medaka. Gene Expr. Patterns. 10, (283-289). 2010.
• Mita, M., Yoshikuni, M., Ohno, K., Shibata, Y., Paul-Prasanth, B., Pichayawasin, S., Isobe M., Nagahama, Y. A relaxin-like peptide purified from radial nerves induces oocyte maturation and ovulation in the starfish, Asterina pectinifera. Proc. Natl. Acad. Sci. USA. 106, (9507-9512). 2009.
• Kato, S., Tsurumaru, S., Tage, M., Yamane, T., Shibata, Y., Ohno, K., Fujiwara, A., Yamano, K., Yoshikuni, M. Neural peptides induce oocyte maturation and gamete spawning of sea cucumber, Apostichopus japonicus. Dev. Biol. 326, (169-176). 2009.
• Zhou, L.Y., Wang, D.S., Shibata, Y., Paul-Prasanth, B., Suzuki, A., Nagahama, Y. Characterization, expression and transcriptional regulation of P450c17-I and -II in the medaka, Oryzias latipes. Biochem. Biophys. Res. Comm. 362, (619-625). 2007.
• Shibata, Y., Iwamatsu, T., Oba, Y., Kobayashi, D., Tanaka, M., Nagahama, Y., Suzuki, N., Yoshikuni, M. Identification and cDNA cloning of alveolin, an extracellular metalloproteinase, which induces chorion hardening of medaka (Oryzias latipes) eggs upon fertilization. J. Biol. Chem. 275, (8349-8354). 2000.

• 日本動物学会
• 日本分子生物学会
• The Society for the Study of Reproduction (SSR)

• Shibata, Y., Zhou, L-Y., Hirai T., Yoshikuni, M., Nagahama, Y. Changes in steroidogenic enzyme, steroidogenic acute regulatory protein, steroid receptor, gonadotropin receptor and transcriptional factor mRNAs in ovarian follicles during oocyte growth and maturation of medaka (Oryzias latipes). 8th International Symposium on Reproductive Physiology of Fish. Saint Malo (France). June 3-8, 2007.
• Shibata, Y. An astacin-like protease, alveolin, released from cortical alveoli, induces the chorion hardening during fertilization in medaka (Oryzias latipes) eggs. The third International Symposium on The Molecular and Cellular Biology of Egg and Embryo Extracellular Matrix. Lake Tohoe (USA). August 27-September 1, 2000.
• 柴田安司，長濱嘉孝. メダカ卵成熟過程における遺伝子発現解析. 日本動物学会第81回大会 (東京). 2009年9月22-25日.

• 柴田安司，長濱嘉孝. メダカ卵成熟過程における遺伝子発現解析. 日本動物学会第81回大会 (東京). 2009年9月22-25日.
• 柴田安司, 荻原克益, 高橋孝行, 吉国通庸, 長濱嘉孝.　メダカ卵濾胞の成長・成熟期におけるGTH受容体、ステロイド関連遺伝子の発現変化. 日本動物学会第78回大会 (弘前). 2007年9月20-22日.
• 柴田安司, 長濱嘉孝, 吉国通庸.　新規膜結合型プロゲスチン受容体. 日本動物学会中部支部大会 (静岡). 2004年7月23-24日.
• 柴田安司, 長濱嘉孝, 吉国通庸.　メダカ卵受精後の卵膜の変化について. 日本動物学会第73回大会 (金沢). 2002年9月24日.
• 柴田安司, 岩松鷹司, 吉国通庸, 大場裕一, 小林大介, 田中実, 長濱嘉孝, 鈴木範男. メダカ卵から受精時に分泌される卵膜硬化酵素の精製と同定.　日本動物学会第70回大会 (山形). 1999年9月27-29日.

• Shibata, Y. Gene Expression profiling of ovarian follicles in medaka, Oryzias latipes, during oocyte maturation. SSR 2014. Grand Rapids (MI, USA), 2014.
• Shibata, Y., Young, G., Nagahama, Y. Gene expression profiling of ovarian follicles in medaka, Oryzias latipes during oocyte maturation. SSR 46th Annual Meeting. Montreal (Canada), July 22-26, 2013.
• Shibata, Y., Young, G., Nagahama, Y., Microarray Analysis of gene expression in ovarian follicles of medaka, Oryzias latipes, during vitellogenesis, maturation and ovulation. 17th Annual Center for Reproductive Biology Retreat. Pullman (WA, USA). 2013.
• Shibata, Y., Nagahama, Y., Young, G. and Naruse, K. Gene expression profiling of ovarian follicles in medaka, Oryzias latipes. 15th Annual Center for Reproductive Biology Retreat. Pullman (WA, USA). May 19, 2011.
• Suzuki, A., Shibata, Y., Matsuda, M., Paul-Prasanth, B., Nagahama, Y. Expression of gonadal soma derived factor (GSDF) is closely correlated with male sex-differentiation in Medaka. 16th International Congress of Comparative Endocrinology. Hong Kong S.A.R. (China). June 22-26, 2009.
• Suzuki, A., Shibata, Y., Paul-Prasanth, B., Nagahama, Y. Expression of gonadal soma derived factor (GSDF) is closely correlated with male sex-differentiation in Medaka. 5th Annual International Symposium on Vertebrate Sex Determination Conference. Kailua-Kona (HI, USA). April 20-24, 2009.
• Shibata, Y., Hirai, T., Yoshikuni, Y., Nagahama, Y. Hormonal Regulation of ovulation in medaka, Oryzias latipes. International Symposium on Sex Determination and Gametogenesis in Fish: Current Status and Future Directions. Honolulu (HI, USA). May 30-June 1, 2008.
• Shibata, Y., Zhou, L-Y., Hirai T., Yoshikuni, M., Nagahama, Y. Changes in steroidogenic enzyme, steroidogenic acute regulatory protein, steroid receptor, gonadotropin receptor and transcriptional factor mRNAs in ovarian follicles during oocyte growth and maturation of medaka (Oryzias latipes). 8th International Symposium on Reproductive Physiology of Fish. Saint Malo (France). June 3-8, 2007.
• Shibata, Y., Nagahama, Y., Yoshikuni, M. The hardening of the egg envelope following fertilization is caused by cortical granule-derived protease in medaka fish. The 4th International Symposium on the Molecular and Cell Biology of Egg- and Embryo-Coats, Mie (Japan) November 8-13, 2004.
• Shibata, Y., Ohno, K., Thomas, P., Nagahama, Y., Yoshikuni, M. Multiple expression of membrane progestin receptors in medaka (Oryzias latipes) ovary. Third International Meeting. Rapid Responses to Steroid Hormones. Florence (Italy). September 12-14, 2003.
• Shibata, Y., Nagahama, Y., Yoshikuni, M. An astacin-like protease, alveolin, is released from cortical vesicles and induces changes in egg envelope proteins during fertilization of medaka (Oryzias latipes) eggs. 7th International Symposium on Reproductive Physiology of Fish. Mie (Japan). May 18-23, 2003.
• Shibata, Y., Nagahama, Y., Yoshikuni, M. Alveolin, an astacin like protease, is exocytozed from cortical vesicles during fertilization in medaka (Oryzias latipes) eggs. 14th International Congress of Comparative Endocrinology, Sorrento (Italy). May 26-30, 2001.
• Shibata, Y. An astacin-like protease, alveolin, released from cortical alveoli, induces the chorion hardening during fertilization in medaka (Oryzias latipes) eggs. The third International Symposium on The Molecular and Cellular Biology of Egg and Embryo Extracellular Matrix. Lake Tohoe (USA). August 27-September 1, 2000.
• Shibata, Y., Iwamatsu, T., Suzuki, N. Changes in chorion proteins induced by the exudate released from the cortex of fish Eggs during fertilization. The Second International Symposium on the Molecular and Cell Biology of Egg- and Embryo-Coats. Sapporo (Japan). October 12-17, 1997.